Irreversible UV-induced quercetin and rutin degradation in solution, studied by UV-spectrophotometry and HPLC chromatography

Comparing the Efficiency of UV/TiO2 and UV/O3 Processes in Degradation of Sulfonamide Antibiotics in Aquatic Solution

Background and purpose: Emerging pollutants such as antibiotics are resistant to biodegradation. The aim of this study was to compare the effect of photocatalytic and Ozonation photolysis on decomposition of Sulfonamide antibiotics (Sulfacetamide, Sulfathiazole, Sulfamethoxazole, and Sulfadiazine) in aquatic environments. Materials and methods: In this study, experiments were conducted discont...

Determination of diclofenac released from suppositories using UV spectrophotometry, spectra derivative spectrophotometry and HPLC.

Spectrophotometric method is often recommended in dissolution testing of not only tablets or capsules but also suppositories. Dissolution rate of diclofenac sodium was studied using a pharmacopoeial flow-through apparatus and pH 7.3 buffer. For two lipophilic suppository bases (Witepsol W-32 and H-19) dissolution rate was overestimated with the spectrophotometric zero-order method in comparison...

Determination and Validation of Zonisamide and its Four Related Substances by HPLC and UV-Spectrophotometry

A selective and sensitive liquid chromatographic method has been developed for simultaneous determination of zonisamide and its four related substances in pharmaceutical dosage forms. The assay involved an isocratic elution in perfectsil Target C18 column using a mobile phase composition of disodium hydrogen phosphate buffer, acetonitrile and methanol (650:150:200 v/v, pH adjusted to 3±0.05) wi...

Basic UV/Visible Spectrophotometry

Determination of Sulfamethoxazole and Trimethoprim in Pharmaceuticals by Visible and UV Spectrophotometry

A method for the simultaneous determination of sulfamethoxazole (SMX) and trimethoprim (TMP), based on a direct determination of SMX after diazotization and coupling with 2-naphthol by visible spectrophotometry and an indirect determination of TMP in the UV region by difference was developed. By the suggested procedure, it was possible to analyze SMX and TMP in pharmaceutical preparations witho...